Phase angle spectroscopy on transparent conducting polymer electrodes for real-time measurement of epithelial barrier integrity
By Loffler, S. & Richter-Dahlfors, A.
Published in J. Mater. Chem. B
The Royal Society of Chemistry
2015
Abstract
To facilitate automated real-time monitoring of the formation or disruption of an epithelial monolayer, phase angle spectroscopy was performed in custom-designed cell culture dishes including a transparent conductive polymer-based 2-electrode device. A peak in [small phi] amplitude at 103-105 Hz specifically correlated to increased capacitance by proliferating cells. Dynamic monitoring of cell density, and barrier integrity during monolayer formation and disruption were investigated in combination with live-cell microscopy.
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