A novel silanization agent based single used biosensing system: Detection of C-reactive protein as a potential Alzheimer
By Karabo?a, M
Published in Journal of Pharmaceutical and Biomedical Analysis
2018
Abstract
This paper illustrates a new and sensitive electrochemical immunosensor for the analysis of C-reactive protein. Indium Tin Oxide (ITO) disposable sheets were modified by using 3-cyanopropyltrimethoxysilane (CPTMS) self-assembled monolayers (SAMs) for the first time for immobilizing the anti-CRP antibody via covalent interactions without the need for any cross-linking agent. Cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS), as well as square wave voltammetry (SWV) methods were applied to characterize immobilization steps of anti-CRP and to determine the CRP concentration. The optimization of the fabricated parameters and the analytical performance of the biosensor were widely evaluated. Charge transfer resistance changes were highly linear and sensitive with CRP concentration of 3.25
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