L-lactate selective impedimetric bienzymatic biosensor based on lactate dehydrogenase and pyruvate oxidase
By Chan, Deni; Barsan, Madalina M.; Korpan, Yaroslav; Brett, Christopher M. A.
Published in Electrochimica Acta
NULL
2017
Abstract
Abstract A new L-lactate selective impedimetric biosensor based on a lactate dehydrogenase (LDH)/pyruvate oxidase (PyrOx) bioselective membrane is reported, fabricated using enzyme drop coating and cross-linking via glutaraldehyde vapours. The sensing strategy is based on the measurement of the change in impedance caused by the generation of charged ions (CH3COO?, H+ and HCO3?), from PyrOx catalysed pyruvate oxidation, the pyruvate resulting from L-lactate oxidation catalysed by {LDH} + nicotinamide adenine dinucleotide (NAD+). The experimental conditions, pH and buffer concentration, were optimized for best biosensor response, and operational/storage stability and selectivity/specificity were investigated. The limit of detection for L-lactate, was 17 and 20 ?M, considering changes in resistance and charge separation parameters at and close to the LDH-NAD+/PyrOx interface, respectively. The biosensor was successfully employed for the determination of L-lactate in yogurt samples.
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