Electrochemical immunosensor based on poly (3,4-ethylenedioxythiophene) modified with gold nanoparticle to detect aflatoxin {B1}
By Sharma, Arati; Kumar, A.; Khan, Raju
Published in Materials Science and Engineering: C
NULL
2017
Abstract
Abstract Gold nanoparticles (Au-NPs) functionalized Poly (3,4-ethylenedioxythiophene) (PEDOT) bioelectrodes were fabricated layer by layer deposition on {ITO} electrode for detection of aflatoxin B1. The monoclonal anti-aflatoxin antibodies (Anti-AFB1) were immobilized over the surface of PEDOT/Au-NPs/ITO using EDC/NHS coupling. The surface morphology and characteristics of the modified electrodes were investigated by scanning electron microscope and contact angle measurements. The electrochemical analysis of the fabricated immunoelectrode and the immobilization of the antibodies have been evaluated and confirmed by performing Cyclic Voltammetry (CV), Electrochemical Impedance Spectroscopy (EIS) and Fourier Transform Infrared Spectroscopy (FTIR). Decrease in the value of electron transfer resistance (Ret) and increase in the peak current values after incorporation of Au-NPs signifies the enhanced properties of {PEDOT} embedded Au-NPs. The heterogeneous rate constant (ks) and transfer coefficient (?) have been determined by using Laviron's method. The fabricated immunosensor exhibits high sensitive amperometric response of 3.72 ?A ng mL? 1 towards {AFB1} concentration in a linear range of 1
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