An electrochemical {DNA} biosensor based on Ni doped ZnO thin film for meningitis detection
By Tak, Manvi; Gupta, Vinay; Tomar, Monika
Published in Journal of Electroanalytical Chemistry
NULL
2017
Abstract
Abstract Nickel doped zinc oxide (Ni-ZnO) thin film based matrix is used for fabricating highly stable and sensitive {DNA} biosensor for detecting a life-threatening disease, Meningitis. The present {DNA} biosensor has been fabricated after the immobilization of 23mer oligonucleotide sequence of {DNA} over the surface of Ni-ZnO/ITO electrode via. electrostatic interaction. Scanning electron microscopy (SEM) studies show the formation of nanostructured Ni doped ZnO thin film surface morphology which facilitates higher loading of single stranded thiolated {DNA} (ss th-DNA) molecules over the Ni doped ZnO matrix. The ss th-DNA/Ni-ZnO/ITO bioelectrode response studies were done using differential pulsed voltammetry (DPV) in the methylene blue (MB) mediated buffer. Linear response over wide {DNA} concentration (5 ng/?l
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