A wireless point-of-care testing system for the detection of neuron-specific enolase with microfluidic paper-based analytical devices

By Fan, Yan; Liu, Juntao; Wang, Yang; Luo, Jinping; Xu, Huiren; Xu, Shengwei; Cai, Xinxia
Published in Biosensors and Bioelectronics NULL 2017

Abstract

Abstract Neuron-specific enolase (NSE) had clinical significance on diagnosis, staging, monitoring effect and judging prognosis of small cell lung cancer. Thus, there had a growing demand for the on-site testing of NSE. Here, a wireless point-of-care testing (POCT) system with electrochemical measurement for {NSE} detection was developed and verified. The wireless {POCT} system consisted of microfluidic paper-based analytical devices (?PADs), electrochemical detector and Android's smartphone. Differential pulse voltammetry (DPV) measurement was adopted by means of electrochemical detector which including a potentiostat and current-to-voltage converter. ?PADs were modified with nanocomposites synthesized by Amino functional graphene, thionine and gold nanoparticles (NH2-G/Thi/AuNPs) as immunosensors for {NSE} detection. Combined with ?PADs, the performance of the wireless {POCT} system was evaluated. The peak currents showed good linear relationship of the logarithm of {NSE} concentration ranging from 1 to 500 ng mL?1 with the limit of detection (LOD) of 10 pg mL?1. The detection results were automatically stored in {EEPROM} memory and could be displayed on Android's smartphone through Bluetooth in real time. The detection results were comparable to those measured by a commercial electrochemical workstation. The wireless {POCT} system had the potential for on-site testing of other tumor markers.

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