A novel label free immunosensor based on single-use ITO-PET electrodes for detection {MAGE1} protein
Published in Journal of Electroanalytical Chemistry NULL 2017
Abstract
Abstract This study is aimed to design a label free biosensor based on transparent ITO-PET (Indium tin oxide- polyethylene terephthalate) electrode to determine {MAGE1} protein. Firstly, indium tin oxide (ITO) electrodes were modified with NH4OH/H2O2/H2O to obtain the {OH} groups on the surface. Later, the surface of {ITO} electrodes were treated with 3-glycidoxypropyltriethoxysilane (3-GOPE). After {SAM} formation, anti- {MAGE1} protein was covalently immobilized on modified {ITO} electrodes. The parameters such as {SAMs} concentration, the concentration and incubation time of anti-MAGE1 solution were optimized. For determining the immobilization steps and optimization of the biosensor, electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were utilized. To determine analytical characterization of designed biosensor; linear range, repeatability, reproducibility, regeneration processes were studied. In optimum conditions, the linear range of the biosensor was determined as 0.01 pg
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