A microbial electrode based on the co-electrodeposition of carboxyl graphene and Au nanoparticles for {BOD} rapid detection

By Li, Yijin; Sun, Jizhou; Wang, Jinfen; Bian, Chao; Tong, Jianhua; Li, Yang; Xia, Shanhong
Published in Biochemical Engineering Journal NULL 2017

Abstract

Abstract Biochemical oxygen demand (BOD) is an international regulatory index for evaluating organic water pollution, which requires to be rapidly detected. In this paper, a microbial electrode for rapid {BOD} sensing has been developed. Carboxyl graphene (GN-COOH) and Au nanoparticles (AuNPs) have been co-electrodeposited on the electrode and microorganisms (Bacillus subtilis) have been immobilized onto the modified electrode through covalent bonding. The modification process has been investigated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), scanning electron microscope (SEM), fluorescence microscope and Fourier transform infrared spectroscopy (FT-IR). The AuNPs with an average particle size of 30 nm are dispersed in the GN-COOH film. The co-electrodeposited film not only facilitates the electron transfer between electrode and reagents, but also creates a biocompatible microenvironment and a rough surface with more sites for cell immobilization. Compared with the traditional {BOD} electrode including immobilized microbial film and oxygen permselective membrane, the B. subtilis/rGN-COOH-AuNPs/Au electrode simplifies the structure for more effective mass transfer. After modification parameters

Read » Back