Fluorescence quenching/enhancement surface assays: Signal manipulation using silver-coated gold nanoparticles
By Matveeva, Evgenia G.; Shtoyko, Tanya; Gryczynski, Ignacy; Akopova, Irina & Gryczynski, Zygmunt
Published in Chemical Physics Letters
NULL
2008
Abstract
Gold nanoparticles covalently attached to the indium tin oxide coated glass slide drastically quench fluorescence of a surface immunoassay (approximately 5-fold). Silver electrochemically deposited over the gold particles leads to fluorescence amplification: signal increases approximately 7–8 times if compared to the signal on gold particles not covered with silver. This phenomenon allows enhancing of the surface immunoassays utilizing both types of nanoparticles.
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