A silicon based implantable microelectrode array for electrophysiological and dopamine recording from cortex to striatum in the non-human primate brain

By Zhang, Song; Song, Yilin; Wang, Mixia; Zhang, Zhiming; Fan, Xinyi; Song, Xianteng; Zhuang, Ping; Yue, Feng; Chan, Piu; Cai, Xinxia
Published in Biosensors and Bioelectronics NULL 2016

Abstract

Abstract Dual-mode, multielectrode recordings have become routine in rodent neuroscience research and have recently been adapted to the non-human primate. However, robust and reliable application of acute, multielectrode recording methods in monkeys especially for deep brain nucleus research remains a challenge. In this paper, We described a low cost silicon based 16-site implantable microelectrode array (MEA) chip fabricated by standard lithography technology for in vivo test. The array was 25 mm long and designed to use in non-human primate models, for electrophysiological and electrochemical recording. We presented a detailed protocol for array fabrication, then showed that the device can record Spikes, {LFPs} and dopamine (DA) variation continuously from cortex to striatum in an esthetized monkey. Though our experiment, high-quality electrophysiological signals were obtained from the animal. Across any given microelectrode, spike amplitudes ranged from 70 to 300 ?V peak to peak, with a mean signal-to-noise ratio of better than 5:1. Calibration results showed the {MEA} probe had high sensitivity and good selectivity for DA. The {DA} concentration changed from 42.8 to 481.6 ?M when the {MEA} probe inserted from cortex into deep brain nucleus of striatum, which reflected the inhomogeneous distribution of {DA} in brains. Compared with existing methods allowing single mode (electrophysiology or electrochemistry) recording. This system is designed explicitly for dual-mode recording to meet the challenges of recording in non-human primates.

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