Ultra-sensitive detection of bacterial toxin with silicon nanowire transistor
By Mishra, Nirankar N.; Maki, Wusi C.; Cameron, Eric; Nelson, Ron; Winterrowd, Paul; Rastogi, Shiva K.; Filanoski, Brian & Maki, Gary K.
Published in Lab Chip
The Royal Society of Chemistry
2008
Abstract
Nanowire field effect transistors (nano-FET) were lithographically fabricated using 50 nm doped polysilicon nanowires attached to two small gold terminals separated from each other by a [similar]150 nm gap to serve as the basis for electronic detection of bacteria toxins. The device characterizations, semiconducting properties and use in a robust and sensitive bio-molecular detection sensor of bacterial toxins were reported in this work. The device characteristics were demonstrated with varying gate and drain voltages. The bio-molecular detection was demonstrated using electrochemical impedance spectroscopy (EIS), using Staphylococcus aureus Enterotoxin B (SEB) as the target molecule. The detection limit of SEB was observed in the range of 10-35 fM.
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