Catalytic reduction of dioxygen with modified Thermus thermophilus cytochrome c552
By Husband, Jonathan; Aaron, Michael S.; Bains, Rajneesh K.; Lewis, Andrew R.; Warren, Jeffrey J.
Published in Journal of Inorganic Biochemistry
NULL
2016
Abstract
Abstract Efficient catalysis of the oxygen reduction reaction (ORR) is of central importance to function in fuel cells. Metalloproteins, such as laccase (Cu) or cytochrome c oxidase (Cu/Fe-heme) carry out the 4H+/4e? reduction quite efficiently, but using large, complex protein frameworks. Smaller heme proteins also can carry out ORR, but less efficiently. To gain greater insight into features that promote efficient ORR, we expressed, characterized, and investigated the electrochemical behavior of six new mutants of cytochrome c552 from Thermus thermophilus: V49S/M69A, V49T/M69A, L29D/V49S/M69A, P27A/P28A/L29D/V49S/M69A, and P27A/P28A/L29D/V49T/M69A. Mutation to {V49} causes only minor shifts to FeIII/II reduction potentials (E
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