Combined surface acoustic wave and surface plasmon resonance measurement of collagen and fibrinogen layer physical properties
By Friedt, J.-M.; Francis, L. A.
Published in Sensing and Bio-Sensing Research
NULL
2016
Abstract
Abstract We use an instrument combining optical (surface plasmon resonance) and acoustic (Love mode surface acoustic wave device) real-time measurements on a same surface for the identification of water content in collagen and fibrinogen protein layers. After calibration of the surface acoustic wave device sensitivity by copper electrodeposition and surfactant adsorption, the bound mass and its physical properties - density and optical index - are extracted from the complementary measurement techniques and lead to thickness and water ratio values compatible with the observed signal shifts. Such results are especially usefully for protein layers with a high water content as shown here for collagen on an hydrophobic surface. We obtain the following results: collagen layers include 70
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