A novel immunosensor based on fullerene {C60} for electrochemical analysis of heat shock protein 70
By Demirbakan, Bur
Published in Journal of Electroanalytical Chemistry
NULL
2016
Abstract
Abstract This study illustrates the development of the biosensor for the determination of the heat shock protein 70 (HSP70). For this purpose, firstly the biosensor based on the glassy carbon electrode (GCE) was modified with fullerene C60. The anti-HSP70 was bound on fullerene {C60} layer by using the EDC/NHS couple. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) methods were applied to characterize the immobilization of anti-HSP70 process and to determine the HSP70. In order to clarify the binding characterization of {HSP70} and anti-HSP70, a single frequency impedance method was utilized, whereas the scanning electron microscopy was used to identify the surface morphology and Kramers-Kronig transform was implemented on impedance data. The biosensor exhibited good repeatability and reproducibility. The linear detection range of the developed biosensor was of 0.8 pg-12.8 pg/mL. Real human blood serum samples were analyzed by the biosensor and the results were also validated by using a reference method based on ELISA.
Microsoft is Ending Support for Windows 7
Microsoft will discontinue support for Windows 7 on January 14, 2020 which means Gamry will also be discontinuing support for Windows 7. If you are upgrading to Windows 10, like many companies and institutions, you need to be running Version 7 of our s oftware . Please note that only USB and Ethernet-based instruments can run in Version 7. Eligible users can download the latest version of our software through our online Client Portal .
If you haven't already registered your instrument, you can do so through the Client Portal .
Please email Technical Support if you have any questions regarding this transition. Please be sure to include your instrument model and serial number when contacting us.