A ZnO-CNT nanocomposite based electrochemical DNA biosensor for meningitis detection
By Tak, Manvi; Gupta, Vinay; Tomar, Monika
Published in RSC Adv
The Royal Society of Chemistry
2016
Abstract
A zinc oxide (ZnO) and multiwalled carbon nanotube (CNT) based nanocomposite matrix has been fabricated onto indium tin oxide (ITO) coated glass (ITO/glass) platform via. A chemical route. The thiolated oligonucleotides probe sequence consisting of 23 bases (single-stranded DNA) has been immobilized onto the ZnO-CNT/ITO electrode using a physical adsorption technique. The fabricated bioelectrode (ssDNA/ZnO-CNT/ITO) is used to specifically detect N. meningitidis with a high sensitivity of 20.20 [small mu ]A per decade and 45 s of hybridization time by differential pulse voltammetry (DPV) using methylene blue as an electroactive indicator. The prepared DNA biosensor exhibits linearity in a wide range (5-180 ng [small mu ]l-1). The electrochemical impedance response behaviour of the ssDNA/ZnO-CNT/ITO bioelectrode with an increase in the target DNA concentration has also been studied using [Fe(CN)6]3-/4- as the hybridization indicator. The results indicate the possibility of fabricating a handheld device for meningitis detection at an early stage.
Microsoft is Ending Support for Windows 7
Microsoft will discontinue support for Windows 7 on January 14, 2020 which means Gamry will also be discontinuing support for Windows 7. If you are upgrading to Windows 10, like many companies and institutions, you need to be running Version 7 of our s oftware . Please note that only USB and Ethernet-based instruments can run in Version 7. Eligible users can download the latest version of our software through our online Client Portal .
If you haven't already registered your instrument, you can do so through the Client Portal .
Please email Technical Support if you have any questions regarding this transition. Please be sure to include your instrument model and serial number when contacting us.