Impedimetric immunosensor for detection of cardiovascular disorder risk biomarker
By Raju Khan and Mintu Pal and Alexey V. Kuzikov and Tanya Bulko and Elena V. Suprun and Victoria V. Shumyantseva
Published in Materials Science and Engineering: C
NULL
2016
Abstract
We report the construction and characterization of a novel, level free impedimetric immunosensor for rapid, sensitive and selective detection of myoglobin (Mb). Monoclonal anti-myoglobin (anti-Mb-IgG) antibody was immobilized on screen-printed multiwalled carbon nanotubes electrode for signal amplification without the need of natural enzymes. The fabrication of resulting immunosensor was extensively characterized by using scanning electron microscopy (SEM), fourier transform infrared (FT-IR) spectroscopy, cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). Electrochemical impedance spectroscopy (EIS) technique offered a linear detection range (0.1
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