Molecularly imprinted polymer film interfaced with Surface Acoustic Wave technology as a sensing platform for label-free protein detection
By Aleksei Tretjakov and Vitali Syritski and Jekaterina Reut and Roman Boroznjak and Andres
Published in Analytica Chimica Acta
NULL
2016
Abstract
Molecularly imprinted polymer (MIP)-based synthetic receptors integrated with Surface Acoustic Wave (SAW) sensing platform were applied for the first time for label-free protein detection. The ultrathin polymeric films with surface imprints of immunoglobulin G (IgG-MIP) were fabricated onto the multiplexed \SAW\ chips using an electrosynthesis approach. The films were characterized by analyzing the binding kinetics recorded by \SAW\ system. It was revealed that the capability of IgG-MIP to specifically recognize the target protein was greatly influenced by the polymer film thickness that could be easily optimized by the amount of the electrical charge consumed during the electrodeposition. The thickness-optimized IgG-MIPs demonstrated imprinting factors towards IgG in the range of 2.8
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