Metabolically redirected biohydrogen pathway integrated with biomethanation for improved gaseous energy recovery

By Shantonu Roy and Debopam Banerjee and Mainak Dutta and Debabrata Das
Published in Fuel NULL 2015

Abstract

Chemical mutagenesis approach was used for the improvement of \H2\ yield using Thermoanaerobacterium thermosaccharolyticum. A \H2\ yield of 3.12 mol mol?1 glucose was observed which was about 13.5% higher as compared to wild type. An inverse relation was observed between the NADH/NAD+ ratio and the rate of \H2\ production. Bioelectrochemical analysis showed an overall increase in reducing equivalent in mutants. Continuous \H2\ production with mutant using starchy wastewater showed maximum \H2\ production rate of 500 mL L?1 h?1 at a dilution rate of 0.25 h?1 and organic loading rate (OLR) of 8.75 g L?1 h?1. The spent medium was mostly comprised of acetate (0.62

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