AuNPs modified, disposable, \ITO\ based biosensor: Early diagnosis of heat shock protein 70
By M
Published in Biosensors and Bioelectronics
NULL
2016
Abstract
This paper describes a novel, simple, and disposable immunosensor based on indium-tin oxide (ITO) sheets modified with gold nanoparticles to sensitively analyze heat shock protein 70 (HSP70), a potential biomarker that could be evaluated in diagnosis of some carcinomas. Disposable \ITO\ coated Polyethylene terephthalate (PET) electrodes were used and modified with gold nanoparticles in order to construct the biosensors. Optimization and characterization steps were analyzed by electrochemical techniques such as electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Surface morphology of the biosensor was also identified by electrochemical methods, scanning electron microscopy (SEM), and atomic force microscopy (AFM). To interpret binding characterization of \HSP70\ to anti-HSP70 single frequency impedance method was successfully operated. Moreover, the proposed \HSP70\ immunosensor acquired good stability, repeatability, and reproducibility. Ultimately, proposed biosensor was introduced to real human serum samples to determine \HSP70\ sensitively and accurately.
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