Simultaneous release of peptides and phenolics with antioxidant, ACE-inhibitory and anti-inflammatory activities from pinto bean (Phaseolus vulgaris L. var. pinto) proteins by subtilisins

By Patricia Garcia-Mora and Juana Frias and Elena Pe
Published in Journal of Functional Foods NULL 2015

Abstract

Production of novel functional ingredients from legume proteins is a valuable strategy to boost their food use and intake. Aimed at a better exploitation of the functional properties of pinto bean hydrolysates, the effectiveness of two subtilisins (Alcalase and Savinase) for the simultaneous release of bioactive peptides and phenolics with antioxidant, angiotensin I converting enzyme (ACE) inhibitory and anti-inflammatory activities was investigated. A higher proteolytic efficiency was observed for Alcalase that increased the concentration of small peptides in hydrolysates after 120?min. Savinase improved hydroxycinnamic acids and flavonoid contents in hydrolysates after 90?min due to its higher esterase activity. The most active hydrolysates combined potent free radical scavenging (326

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