Catalytic biofilm formation by Shewanella oneidensis MR-1 and anode characterization by expanded uncertainty
By Roy, Jared N.; Babanova, Sofia; Garcia, Kristen E.; Cornejo, Jose; Ista, Linnea K. & Atanassov, Plamen
Published in Electrochimica Acta
NULL
2014
Abstract
In this study, high-density planktonic cultures of Shewanella oneidensis MR-1 are grown aerobically to early stationary phase. After washing the cells and removing from original medium, the culture is exposed to an anaerobic environment in an electrochemical cell. An applied potential of -0.30 V vs Ag/AgCl is applied to the working electrode and the corresponding current is measured via chronoamperometry. Current begins to increase within 2–3 h stabilizing at 5 h. Cyclic voltammetry was measured at 5 h indicating the initial stages a kinetically limited biofilm and again at 24 h with an apparently more stable catalytic biofilm. At this point, the biofilm appears to suffer mass transport limitation as the catalytic wave dominates the shape of the voltammogram, similar to voltammograms reported for Geobacter spp. Polarization curves are also reported herein, further demonstrating a large increase of current near the oxidation potential of what is believed to be the terminal protein complex (MtrC/OmcA) of the trans-membrane cytochrome cascade, the Mtr pathway. Additional characterization and comparison between replicates of the biofilm is made using the idea of expanded uncertainty. This novel approach in reporting measured results for microbial fuel cells elucidates specific electrochemical parameters for appropriate comparison between systems and laboratories.
Microsoft is Ending Support for Windows 7
Microsoft will discontinue support for Windows 7 on January 14, 2020 which means Gamry will also be discontinuing support for Windows 7. If you are upgrading to Windows 10, like many companies and institutions, you need to be running Version 7 of our s oftware . Please note that only USB and Ethernet-based instruments can run in Version 7. Eligible users can download the latest version of our software through our online Client Portal .
If you haven't already registered your instrument, you can do so through the Client Portal .
Please email Technical Support if you have any questions regarding this transition. Please be sure to include your instrument model and serial number when contacting us.