A new strategy for the preparation of maleimide-functionalised gold surfaces
By Zhang, Xin; Sun, Guoguang; Hovestà¤dt, Marc; Syritski, Vitali; Esser, Norbert; Volkmer, Rudolf; Janietz, Silvia; Rappich, Jörg & Hinrichs, Karsten
Published in Electrochemistry Communications
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2010
Abstract
We have developed and investigated a new route to functionalise Au surfaces using maleimide groups. This functionalisation has been performed by grafting aminophenyl (AP) via an electrochemical reduction of 4-aminophenyldiazonium salt in acetonitrile solution and the subsequent chemical binding of N-(2-carboxyethyl) maleimide (NCEM). The resulting maleimide functionalised surface was interacted with a cysteine-modified peptide. The grafting of AP was monitored by the occurrence of NH2 and aryl ring vibrations, whereas the binding of the NCEM led to a strong and sharp peak because of the Câ•?O stretching mode. The immobilisation of the peptide was identified by the appearance of the amide I band. Half of the maleimide surface groups reacted with the peptide because of steric hindrance. The charge efficiency for the AP layer formation was about 45% at a thickness of about 6–8 nm.
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