Two-enzyme lactose biosensor based onβ-galactosidase and glucose oxidase deposited by AC-electrophoresis: Characteristics and performance for lactose determination in milk
By Ammam, Malika & Fransaer, Jan
Published in Sensors and Actuators B: Chemical
NULL
2010
Abstract
In this paper, we have investigated the deposition of two enzymesβ-galactosidase (β-Gal) and glucose oxidase (GOx) using alternating current electrophoretic deposition (AC-EPD) to manufacture a lactose sensor. Using the optimal deposition parameters and optimal testing conditions, the sensor has a sensitivity up to 111 nA/mM mm2. This sensitivity is high considering the low activity of the enzymes employed in this study (9 units/mg forβ-Gal and 5.6 units/mg for GOx). The sensor has a large linear range up to 14 mM lactose, fast response time (∼8 s) and reasonable stability without employing any stabilizers or outer polymer membrane. In addition, it is easy and simple to manufacture, highly reproducible and cheap because low activity enzymes are used. The sensor was tested to determine lactose in milk samples and is demonstrated to be accurate.
Microsoft is Ending Support for Windows 7
Microsoft will discontinue support for Windows 7 on January 14, 2020 which means Gamry will also be discontinuing support for Windows 7. If you are upgrading to Windows 10, like many companies and institutions, you need to be running Version 7 of our s oftware . Please note that only USB and Ethernet-based instruments can run in Version 7. Eligible users can download the latest version of our software through our online Client Portal .
If you haven't already registered your instrument, you can do so through the Client Portal .
Please email Technical Support if you have any questions regarding this transition. Please be sure to include your instrument model and serial number when contacting us.