Insights on the kinetics of concanavalin A adsorption on platinum and glassy carbon electrodes from electrochemical impedance spectroscopy data
By Diniz, Flamarion Borges; Silva, Diego Jos
Published in Colloids and Surfaces A: Physicochemical and Engineering Aspects
2019
Abstract
Electrochemical impedance spectroscopy was applied to investigate the adsorption of concanavalin A on glassy carbon and platinum electrodes. A redox probe, K4Fe(CN)6/K3Fe(CN)6, was used to yield a well defined charge transfer resistance across the electrode/solution interface. Adsorption on glassy carbon electrode was a diffusion controlled process during its initial stages. From the time dependence of the electrode coverage, by means of a simple rate law and the Stoke-Einstein equation, it was possible to estimate a protein effective radius of 3.7 nm and to estimate the protein monolayer coverage of 2 mg m
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